ANALYTICAL PROCEDURE FOR THE DETERMINATION OF LINCOMYCIN AND TYLOSIN ANTIBIOTIC RESIDUES IN MILK BY LIQUID CHROMATOGRAPHY-COUPLED TANDEM MASS SPECTROMETRY (LC–MS/MS)

Alija, Gjylai (2019) ANALYTICAL PROCEDURE FOR THE DETERMINATION OF LINCOMYCIN AND TYLOSIN ANTIBIOTIC RESIDUES IN MILK BY LIQUID CHROMATOGRAPHY-COUPLED TANDEM MASS SPECTROMETRY (LC–MS/MS). Journal of Natural Sciences and Mathematics of UT, 4 (7-8). pp. 41-48. ISSN 2671-3039

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Abstract

A rapid and sensitive liquid chromatography / mass spectrometry (LC–MS/MS) method has been developed and validated for the simultaneous identification, confirmation, and quantitation of tylosin and lincomycin residues in milk. The method was validated in accordance with Commission Decision 2002/657/EC. McIlvaine buffer was used to extract residues from milk, and further SPE cleanup was performed using an Oasis HLB cartridges. Chromatographic separation was achieved using a C18 column (50 x 2.1 mm, 1.7µm) with mobile phase A: 0.1% formic acid in water and mobile phase B: 0.1% formic acid in acetonitrile. The linear regression analysis showed a good correlation with R2 from 0.9879to 0.9978. The recoveries of target compounds were 84.5 % for lincomycin and 92.70 % for tylosin. The decision limits (CCα) for lincomycin was 15.30μg/L and for tylosin 13.50 μg/L. Detection capability (CCβ) was 17.60 μg/L and 16.85 μg/L, respectively. From the validation study results can conclude that the method is suitable to be applied for routine analysis of the residues of lincomycin and tylosin in milk.

Item Type:	Article
Subjects:	Q Science > Q Science (General)
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User:	Unnamed user with email zshi@unite.edu.mk
Date Deposited:	22 Feb 2020 18:39
Last Modified:	22 Feb 2020 18:39
URI:	http://eprints.unite.edu.mk/id/eprint/435

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